RNA family
In molecular biology SprD (S mall p athogenicity island R NA D ) is a non-coding RNA expressed on pathogenicity islands in Staphylococcus aureus [ 1] in silico microarray analysis which were confirmed using a Northern blot .[ 2] disease in an animal model .[ 1]
Function
SprD is located between genes scn and chp in the innate immune evasion cluster (IEC) of the S. aureus genome . Its placement within this region was the first indication of a virulence -factor regulatory function.[ 1]
SprD binds with sbi (Staphylococcus aureus binder of IgG )[ 3] mRNA which encodes an immune evasion protein. It occludes the Shine-Dalgarno sequence and the initiation codon of sbi , forming a sbi mRNA-SprD duplex repressing the translation of the mRNA.[ 1]
sbi protein interferes with the host's innate immune response by binding Factor H , Complement component 3 and IgG .[ 3] [ 4]
See also
References
^ a b c d Chabelskaya S, Gaillot O, Felden B (June 2010). "A Staphylococcus aureus small RNA is required for bacterial virulence and regulates the expression of an immune-evasion molecule" . PLOS Pathog . 6 (6): e1000927. doi :10.1371/journal.ppat.1000927 PMC 2880579 PMID 20532214 . {{cite journal }}: CS1 maint: article number as page number (link )^ Pichon C, Felden B (October 2005). "Small RNA genes expressed from Staphylococcus aureus genomic and pathogenicity islands with specific expression among pathogenic strains" . Proc. Natl. Acad. Sci. U.S.A . 102 (40): 14249– 14254. doi :10.1073/pnas.0503838102 PMC 1242290 PMID 16183745 . ^ a b Haupt K, Reuter M, van den Elsen J, et al. (December 2008). "The Staphylococcus aureus protein Sbi acts as a complement inhibitor and forms a tripartite complex with host complement Factor H and C3b" . PLOS Pathog . 4 (12): e1000250. doi :10.1371/journal.ppat.1000250 PMC 2602735 PMID 19112495 . {{cite journal }}: CS1 maint: article number as page number (link )^ Zhang L, Jacobsson K, Vasi J, Lindberg M, Frykberg L (April 1998). "A second IgG-binding protein in Staphylococcus aureus" . Microbiology . 144 (4): 985– 991. doi :10.1099/00221287-144-4-985 PMID 9579072 . Retrieved 2010-08-09 .
Further reading
Upadhyay A, Burman JD, Clark EA, et al. (August 2008). "Structure-function analysis of the C3 binding region of Staphylococcus aureus immune subversion protein Sbi" . J. Biol. Chem . 283 (32): 22113– 22120. doi :10.1074/jbc.M802636200 PMC 2494919 PMID 18550524 . Burman JD, Leung E, Atkins KL, et al. (June 2008). "Interaction of human complement with Sbi, a staphylococcal immunoglobulin-binding protein: indications of a novel mechanism of complement evasion by Staphylococcus aureus" . J. Biol. Chem . 283 (25): 17579– 17593. doi :10.1074/jbc.M800265200 PMC 2649420 PMID 18434316 . Atkins KL, Burman JD, Chamberlain ES, et al. (March 2008). "S. aureus IgG-binding proteins SpA and Sbi: host specificity and mechanisms of immune complex formation". Mol. Immunol . 45 (6): 1600– 1611. doi :10.1016/j.molimm.2007.10.021 . PMID 18061675 . Chabelskaya S, Bordeau V, Felden B (Apr 1, 2014). "Dual RNA regulatory control of a Staphylococcus aureus virulence factor" . Nucleic Acids Research . 42 (8): 4847– 4858. doi :10.1093/nar/gku119 . PMC 4005698 PMID 24510101 .
External links
