Difructose-anhydride synthase

In enzymology, a difructose-anhydride synthase (EC 3.2.1.134) is an enzyme that catalyzes the chemical reaction

bis-D-fructose 2',1:2,1'-dianhydride + H₂O ${\displaystyle \rightleftharpoons }$ inulobiose

Thus, the two substrates of this enzyme are bis-D-fructose 2',1:2,1'-dianhydride and H₂O, whereas its product is inulobiose.

This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is bis-D-fructose 2',1:2,1'-dianhydride fructohydrolase. This enzyme is also called inulobiose hydrolase.

• Matsuyama T; Tanaka K (1989). "On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobiose". Agric. Biol. Chem. 53 (3): 831–832. doi:10.1271/bbb1961.53.831.
• Matsuyama T, Tanaka K, Mashiko M, Kanamoto M (October 1982). "Enzymic formation of di-D-fructose 1,2'; 2,1' dianhydride from inulobiose by Aspergillus fumigatus". J. Biochem. 92 (4). Tokyo: 1325–8. doi:10.1093/oxfordjournals.jbchem.a134051. PMID 6757245.

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