RNASEH1

Ribonukleaza H1
	; PDB prikaz baziran na 2qk9.
Dostupne strukture
	2QK9, 2QKB, 2QKK, 3BSU
Identifikatori
Simboli	RNASEH1; H1RNA; RNH1
Vanjski ID	OMIM: 604123 MGI: 1335073 HomoloGene: 2202 GeneCards: RNASEH1 Gene
EC broj	3.1.26.4
Ontologija gena
Molekulska funkcija	• vezivanje jona magnezijuma • vezivanje nukleinske kiseline • RNK vezivanje
Ćelijska komponenta	• nukleus • mitohondrija
Biološki proces	• mitohondrijska DNK replikacija • RNK katabolički proces
Ortolozi

edit

Ribonukleaza H1, takođe poznata kao RNaza H1, je enzim, koji je kod ljudi kodiran RNASEH1 genom.^[1]^[2]^[3] RNaza H1 je nespecifična endonukleaza koja katalizuje presecanje RNK hidrolitičkim mehanizmom.

Reference

↑ „Entrez Gene: ribonuclease H1”.
↑ ten Asbroek AL, van Groenigen M, Jakobs ME, Koevoets C, Janssen B, Baas F (June 2002). „Ribonuclease H1 maps to chromosome 2 and has at least three pseudogene loci in the human genome”. Genomics 79 (6): 818–23. DOI:10.1006/geno.2002.6776. PMID 12036296.
↑ Nowotny M, Gaidamakov SA, Ghirlando R, Cerritelli SM, Crouch RJ, Yang W (October 2007). „Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription”. Mol. Cell 28 (2): 264–76. DOI:10.1016/j.molcel.2007.08.015. PMID 17964265.

Literatura

Nowotny M, Cerritelli SM, Ghirlando R, et al. (2008). „Specific recognition of RNA/DNA hybrid and enhancement of human RNase H1 activity by HBD.”. EMBO J. 27 (7): 1172–81. DOI:10.1038/emboj.2008.44. PMC 2323259. PMID 18337749.
Lima WF, Wu H, Nichols JG, et al. (2003). „Human RNase H1 uses one tryptophan and two lysines to position the enzyme at the 3'-DNA/5'-RNA terminus of the heteroduplex substrate.”. J. Biol. Chem. 278 (50): 49860–7. DOI:10.1074/jbc.M306543200. PMID 14506260.
Lima WF, Rose JB, Nichols JG, et al. (2007). „The positional influence of the helical geometry of the heteroduplex substrate on human RNase H1 catalysis.”. Mol. Pharmacol. 71 (1): 73–82. DOI:10.1124/mol.106.025429. PMID 17028157.
Lima WF, Nichols JG, Wu H, et al. (2004). „Structural requirements at the catalytic site of the heteroduplex substrate for human RNase H1 catalysis.”. J. Biol. Chem. 279 (35): 36317–26. DOI:10.1074/jbc.M405035200. PMID 15205459.
Otsuki T, Ota T, Nishikawa T, et al. (2005). „Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.”. DNA Res. 12 (2): 117–26. DOI:10.1093/dnares/12.2.117. PMID 16303743.
Wu H, Lima WF, Crooke ST (1999). „Properties of cloned and expressed human RNase H1.”. J. Biol. Chem. 274 (40): 28270–8. DOI:10.1074/jbc.274.40.28270. PMID 10497183.
Frank P, Braunshofer-Reiter C, Pöltl A, Holzmann K (1998). „Cloning, subcellular localization and functional expression of human RNase HII.”. Biol. Chem. 379 (12): 1407–12. DOI:10.1515/bchm.1998.379.12.1407. PMID 9894807.
Wu H, Lima WF, Zhang H, et al. (2004). „Determination of the role of the human RNase H1 in the pharmacology of DNA-like antisense drugs.”. J. Biol. Chem. 279 (17): 17181–9. DOI:10.1074/jbc.M311683200. PMID 14960586.
Hillier LW, Graves TA, Fulton RS, et al. (2005). „Generation and annotation of the DNA sequences of human chromosomes 2 and 4.”. Nature 434 (7034): 724–31. DOI:10.1038/nature03466. PMID 15815621.
Lima WF, Wu H, Nichols JG, et al. (2003). „Human RNase H1 activity is regulated by a unique redox switch formed between adjacent cysteines.”. J. Biol. Chem. 278 (17): 14906–12. DOI:10.1074/jbc.M211279200. PMID 12473655.
ten Asbroek AL, van Groenigen M, Nooij M, Baas F (2002). „The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides.”. Eur. J. Biochem. 269 (2): 583–92. DOI:10.1046/j.0014-2956.2001.02686.x. PMID 11856317.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). „The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”. Genome Res. 14 (10B): 2121–7. DOI:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). „Complete sequencing and characterization of 21,243 full-length human cDNAs.”. Nat. Genet. 36 (1): 40–5. DOI:10.1038/ng1285. PMID 14702039.
Cerritelli SM, Crouch RJ (1998). „Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI.”. Genomics 53 (3): 300–7. DOI:10.1006/geno.1998.5497. PMID 9799596.
Strausberg RL, Feingold EA, Grouse LH, et al. (2002). „Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.”. Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. DOI:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Cerritelli SM, Frolova EG, Feng C, et al. (2003). „Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice.”. Mol. Cell 11 (3): 807–15. DOI:10.1016/S1097-2765(03)00088-1. PMID 12667461.
Lima WF, Rose JB, Nichols JG, et al. (2007). „Human RNase H1 discriminates between subtle variations in the structure of the heteroduplex substrate.”. Mol. Pharmacol. 71 (1): 83–91. DOI:10.1124/mol.106.025015. PMID 17028158.

[entrez-1] „Entrez Gene: ribonuclease H1”.

[pmid12036296-2] ten Asbroek AL, van Groenigen M, Jakobs ME, Koevoets C, Janssen B, Baas F (June 2002). „Ribonuclease H1 maps to chromosome 2 and has at least three pseudogene loci in the human genome”. Genomics 79 (6): 818–23. DOI:10.1006/geno.2002.6776. PMID 12036296.

[pmid17964265-3] Nowotny M, Gaidamakov SA, Ghirlando R, Cerritelli SM, Crouch RJ, Yang W (October 2007). „Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription”. Mol. Cell 28 (2): 264–76. DOI:10.1016/j.molcel.2007.08.015. PMID 17964265.

[1]

[2]

[3]

RNASEH1

Reference

Literatura

Portal di Ensiklopedia Dunia